The Three Lions kick-off their EURO 2020 qualifying campaign against the Czechs at Wembley on Friday 22 March, before they travel to Podgorica to face Montenegro three days later.
And Southgate’s first selection of 2019 sees an England call-up for West Ham United’s Declan Rice, who only recently switched his international allegiance from the Republic of Ireland.
There is also a return for Burnley goalkeeper Tom Heaton, who is in the squad for the first time since the pre-tournament preparations for last summer’s FIFA World Cup finals.
The squad will get together at St. George's Park to begin their preparations on Monday.
There is still limited availability of tickets for the Czech Republic game in Club Wembley, where membership guarantees your seat for all England men’s senior internationals at Wembley.
And members of the England Supporters Club get priority access to tickets for all of the Three Lions’ home matches, so sign up for free now.
Trent Alexander-Arnold (Liverpool), Ross Barkley (Chelsea), Jack Butland (Stoke City), Ben Chilwell (Leicester City), Dele (Tottenham Hotspur), Fabian Delph (Manchester City), Eric Dier (Tottenham Hotspur), Jordan Henderson (Liverpool), Tom Heaton (Burnley), Harry Kane (Tottenham Hotspur), Michael Keane (Everton), Ruben Loftus-Cheek (Chelsea), Harry Maguire (Leicester City), Jordan Pickford (Everton), Marcus Rashford (Manchester United), Declan Rice (West Ham United), Danny Rose (Tottenham Hotspur), Jadon Sancho (Borussia Dortmund), Luke Shaw (Manchester United), Raheem Sterling (Manchester City), John Stones (Manchester City), James Tarkowski (Burnley), Kieran Trippier (Tottenham Hotspur), Kyle Walker (Manchester City), Callum Wilson (AFC Bournemouth)