National League South's Maidstone United conjured up the magic of the Cup to dump League Two side Cheltenham Town out of the competition in a first round fairy tale.
Delano Sam-Yorke, scorer of three Emirates FA Cup goals in a prior spell at Lincoln City, was chief architect, bagging a double before being sent off in an eventful performance.
Joe Pigott and Zavon Hines were also on target in the 4-2 win, Stones’ maiden appearance in the first round since their reformation in 1992.
Elsewhere, a stirring second-half comeback saw Boreham Wood of the National League knock out former Premier League side Blackpool.
Substitute Dan Holman stabbed home from a yard in the 88th minute to spark pandemonium at Meadow Park - Luke Garrard’s men sealing their first ever win over a league side.
Oxford City, rock bottom of National League South and without a win since August, got in on the upset game with a victory over Colchester United of League Two.
The City toppled the U’s - 56 places above them in the footballing ladder - when Matt Paterson nodded home just after half-time.
Lincoln City won a place in everyone’s hearts with their run to the quarter finals last year but they failed to win a place in the second round this time, their journey ending 1-0 at former winners AFC Wimbledon.
2008 winners Portsmouth fell at the first hurdle for the fifth time in six seasons, pipped 1-0 by Luton Town at Kenilworth Road thanks to James Collins’s strike in first-half added time.
Slough Town hit Gainsborough Trinity for six with Matt Lench remarkably bagging himself a hat-trick after doing exactly the same last week in the Buildbase FA Trophy.
League One’s Blackburn, appearing in the first round for the first time in 37 years, faced humiliation when trailing after an hour, but three late goals took them past Barnet 3-1.
There were to be no fairy tales for Bromley, who lost 4-0 at Rochdale, or Shaw Lane who were seen off 3-1 by Steve Evans’s Mansfield Town in the day’s early kick-off.