With 737 clubs being accepted into the 2020-21 term of the competition, 366 of those will kick off their campaign in the extra preliminary round on Tuesday 1 September.
And there will also be 20 clubs making their debut in the competitions this season, so here are the names to keep an eye out for:
Alfold FC – Based in Surrey, they currently play in the Southern Combination Premier League.
Benfleet FC – The Essex outfit play their games on Canvey Island, where they ground share at Park Lane. They compete in the Eastern Counties League
Billingshurst FC – Orginally established in 1891, the West Sussex club play in the Southern Combination Division One.
Bovey Tracey FC – The Moorlanders play at Mill Marsh Park in their home county of Devon, where they feature in the South West Peninsula League.
Burton Park Wanderers FC – Based in the Northamptonshire town of Burton Latimer, where they play in the United Counties League.
Chelmsley Town FC – The former club of Watford captain and 2019 Cup Finalist Troy Deeney. They play at Pack Meadow, on the outskirts of Solihull.
Hashtag United FC – Founded in 2016 by YouTube star Spencer Owen and with a significant online following, they’ve been on a remarkable climb and only joined the NLS in 2018.
Helston Athletic FC – The Cornish club were founded in 1896 and currently play in the South West Peninsula League Premier West Division.
Kennington FC – Based in Ashford, Kent, they play in the Southern Counties East Division One and share a home with Ashford United at Homelands.
Little Oakley FC – Set in the coastal Essex town of Harwich, this community-based club play their football in the Thurlow Nunn Division One South.
Long Crendon FC – Established in 1896 and based in Buckinghamshire, but play their games at nearby Oxford City’s ground in the Hellenic Football League.
Millbrook FC – Another club from Cornwall making their Cup debut, they play at Jenkins Park in the South West Peninsula League.
New Salamis FC – Former winners of the FA Sunday Cup in 2016, the club were originally set up by members of the Greek-Cypriot community in north east London.
Newark Flowserve FC – Based in the Nottinghamshire market town and formed in 1901, they play in the Midland League Premier Division.
Newent Town FC - The Gloucestershire club play their home games at the gloriously-named Wildsmith Meadow, in the Hellenic League.
Park View FC – The Tottenham club were formed in 1991 as a group of friends forming a Sunday League team and have progressed from there. They play in Wood Green, in north London.
Shelley FC – A community club based in Huddersfield, west Yorkshire. They run a host of teams, but their men’s seniors play in the North West Counties League.
Stansfeld FC – The Southern Counties East team are based at Foxbury Avenue in Chislehurst. Their full name is Stansfeld Oxford & Bermondsey Club Football Club
Westside FC – Oringially formed in Wandsworth, south London, they now play their Combined Counties League games in the more suburban climes of Chessington.
Wythenshawe Amateurs FC – Formed in 1946 as the Wythenshawe Lads Club, the Manchester club play in the North West Counties League.