Kyle Walker has withdrawn from the England squad to face Denmark at Wembley on Wednesday evening.
The Tottenham Hotspur defender reported to the team hotel with an injury on Sunday, but remained with the group on Monday before returning to his club.
"It is unfortunate of course," said England Manager Roy Hodgson.
England v Denmark
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Wembley Stadium connected by EE
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"He hasn't played for the last two or three games and we were hoping he would recover, but he needs at least another week before he will be fit to play.
"He was with us last night, we had a Brazil meeting to put our plans forward."
And with Walker out of the reckoning, Hodgson confirmed that Liverpool's Glen Johnson will win his 50th cap against the Danes, the side he faced on his debut in 2003.
"There aren't that many defenders so there is a good chance [Johnson will play].
"If you insist I tell you Glen Johnson will get his 50th cap at right-back, then I can say yes."
No further replacement for Walker will be called in at this time.